Food safety kits
Enzyme-linked immunosorbent assay established a quantitative ELISA method based on the selectivity and specificity of antibody and antigen recognition. The main principle of ELISA: one of these immune components is fixed in the holes of the solid phase (enzyme plate), and the detected target in the sample interacts with the antibody-antigen system. This interaction can be observed by enzymes in enzyme markers and indicate whether antibody-antigen binding occurs. The added substrate is transformed by coupling enzyme, resulting in color change, which can be measured by Microplate Reader.
Advantages of ELISA
- High sensitivity: ppb(ng/mL) level
- Quantitative analysis and no radioisotope pollution
- Low batch-to-batch variation
- Good specificity
- Long valid period
- Suitable for multiple samples
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