Flow cytometry antibodies

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Flow cytometry
ApplicationAbbreviationDescription
ActivationActivInduce the normal resting state cell to active state.
BlockingBlockUse the cell-surface targeted antibody to block a biological response
Cell SeparationSeparationSeparate the defined cells population using magnetic nanoparticles either by positive ornegative selection.
Cell Separation byNegative SelectionCell Sep-NegSeparate the defined cell population using magnetic nanoparticles by negative selection,also called depletion.
DepletionDepletionIn vivo, refers to injecting the antibody to deplete targeted cell types.In vitro, refers to adding the antibody in culture to deplete target cells.
Functional AssayFAUse the cell-surface targeted antibody to induce, modify, or block a biological response.Can also be applied to use the antibody to measure a functional activity within a cell.
NeutralizationNeutUse the antibody to Neutralize the soluble factor.
StimulationStimUse the antibody to induce the measurable cellular response.
Flow CytometryFCMFlow cytometric analysis of surface-stained cells.
Intracellular Staining forFlow CytometryICFCMFlow cytometric analysis of intracellularly-stained cells.
Western BlottingWBUse the gel electrophoresis to separate proteins by molecular weight, followed bydetectionwith an antibody.
ImmunohistochemistryIHCAnalyze the antibody-labeled tissue preparations, typically frozen or paraffin-embedded.
ImmunofluorescenceIFAnalyze the fluorescently-labeled cells or tissue with the Microscopic examination
ImmunoprecipitationIPUse the antibody to precipitate the target protein from solution.

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